Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEN1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7
cell line
MCF7
cell type
Human breast cancer cell
chip antibody
anti-MEN1 (Bethyl Laboratories A300-115A)
treatment
EPZ

Sequenced DNA Library

library_name
GSM6124956
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated to ~200 bp fragment using a probe sonicator. The DNA fragments were repaired, A-tailed and further ligated with Illumina adapter. The final DNA library was obtained by size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
21667447
Reads aligned (%)
90.5
Duplicates removed (%)
15.9
Number of peaks
937 (qval < 1E-05)

hg19

Number of total reads
21667447
Reads aligned (%)
89.8
Duplicates removed (%)
16.1
Number of peaks
334 (qval < 1E-05)

Base call quality data from DBCLS SRA